Other

Part:BBa_K5427009:Design

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-22)

Linker_2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Given the short length of our linkers, it was necessary to perform complete manual codon optimization. This approach ensured that no repetitive sequences or palindromes were introduced, maintaining sequence stability and preventing unwanted structural formations.


Source

Kolossov, V. L., Spring, B. Q., Sokolowski, A., Conour, J. E., Clegg, R. M., Paul, & H. Rex Gaskins. (2008). Engineering Redox-Sensitive Linkers for Genetically Encoded FRET-Based Biosensors. Experimental Biology and Medicine, 233(2), 238–248. https://doi.org/10.3181/0707-rm-192

References